Here is the latest Iowa news from The Associated Press at 3:45 a.m. CDT

JOHNSTON, Iowa (AP) — At least two school districts in Iowa are refusing to follow the governor’s demand that they return students to classrooms rebuffing the idea that the state can override what local officials believe is the safest way to educate their children as coronavirus spreads in their counties. Gov. Kim Reynolds says the state will require at least half of a school’s instruction to be held in a classroom and the state will decide when K-12 schools can send students home based on community virus spread and student illnesses. Those rejecting the state mandate will not be credited for days of home learning not approved by the state and school administrators may be subject to “licensure discipline.”

LE MARS, Iowa (AP) — An 82-year-old Iowa man has pleaded not guilty to killing his wife’s son during a domestic disturbance at his northwestern Iowa home in May. The Sioux City Journal reports that Thomas Knapp entered the plea Monday in Plymouth County District Court. Knapp is charged with first-degree murder and willful injury in the May 11 death of 51-year-old Kevin Juzek in Knapp’s Merrill home. Police say Knapp shot Juzek with a 20-gauge shotgun through a bedroom door, then deliberately shot him again in the chest.

CHARLES CITY, Iowa (AP) — Authorities say a northeastern Iowa woman has died of her injuries after her bicycle was hit by a truck in rural Floyd County. The Globe Gazette reports that the crash happened Sunday evening, when a truck driven by a 43-year-old Clarksville man, hit a bicycle ridden by 28-year-old Ellen Bengston, of Charles City. The Iowa State Patrol says Bengston was flown to a Mason City hospital. Hospital officials said Monday that Bengston had died of her injuries. The crash remains under investigation.

ANKENY, Iowa (AP) — Police in suburban Des Moines say a pregnant teenager has died in an overnight shooting at an Ankeny hotel. Police say the shooting happened around 11 p.m. Monday at WoodSpring Suites, near Interstate 35. Arriving officers found 17-year-old Mia Holmes, of Ankeny, suffering from a gunshot wound to the chest. Homes was rushed to a local hospital, where she and her unborn child died. Investigators say they are searching for an 18-year-old Des Moines man. Police indicated the shooting was not believed to be random.